Reporter

Part:BBa_K1878000

Designed by: Margaret Lie   Group: iGEM16_Rice   (2016-10-14)


iRFP670

General Properties

iRFP670 is a fluorescent protein that has an excitation wavelength of 643 nm and an emission wavelength of 670 nm. It utilizes billiverdin as a chromophore. The extinction coefficient is 114,000 M-1 cm-1, and the quantum yield is 11.1%. It is twice as bright as iRFP713 (formerly known as iRFP). The photobleaching half-life is 290 s and pKa is 4.0. Near-infrared fluorescent proteins are good for long-term in vivo imaging and exhibit high brightness in bacteria, mammalian cells, and mice.

The original citation is as follows: D.M. Shcherbakova and V.V. Verkhusha. "Near-infrared fluorescent proteins for multicolor in vivo imaging." Nature Methods, 2013. 10(8): p. 751-4.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 81
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 550
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 130
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 636
    Illegal BsaI.rc site found at 193


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Parameters
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